Improved immuno-detection of a low-abundance cyclophilin allows the confirmation of its expression in a protozoan parasite

dc.contributor.author Bustos, Patricia L.
dc.contributor.author Perrone, Alina E
dc.contributor.author Milduberger, Natalia A.
dc.contributor.author Bua, Jaqueline
dc.date.accessioned 2023-10-03T18:33:55Z
dc.date.available 2023-09-27T19:47:54Z
dc.date.available 2023-10-03T18:33:55Z
dc.date.issued 2015-10-6
dc.description.abstract Protein samples can be challenging to analyze due to the presence of high-abundance proteins masking low abundance proteins of interest, such as biomarkers and novel physiological mediators. Cyclophilins are chaperones involved in the cis/trans isomerization of peptidyl-prolyl bonds in peptides or proteins and have been found in every organism sequenced to date. Although considerable progress has been made in the characterization of some cyclophilins expressed in diverse parasites invading humans, the main aspects of low-abundance members of this family remain unknown. In the present work, we present that the combined strategy of using more specific antibodies and increasing the presence of subcellular proteins in the sample, allowed us to confirm the expression of a 21.1 kDa cyclophilin for the first time in Trypanosoma cruzi.
dc.identifier.citation Bustos, P.L.; Perrone, A.E.; Milduberger, N.A.; Bua, J. (2015). Improved immuno-detection of a low-abundance cyclophilin allows the confirmation of its expression in a protozoan parasite. In: Immunochemistry & Immunopathology 1(1):1000103
dc.identifier.other 10.4172/2469-9756.1000103
dc.identifier.uri https://repositorio.uai.edu.ar/handle/123456789/1750.2
dc.language.iso en
dc.publisher Hilaris
dc.subject low-abundance proteins
dc.subject subcellular fractionation
dc.subject anti-synthetic peptides antibodies
dc.subject cyclophilins
dc.subject trypanosoma cruzi
dc.title Improved immuno-detection of a low-abundance cyclophilin allows the confirmation of its expression in a protozoan parasite
dc.type ARTICULO
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