Improved immuno-detection of a low-abundance cyclophilin allows the confirmation of its expression in a protozoan parasite
Improved immuno-detection of a low-abundance cyclophilin allows the confirmation of its expression in a protozoan parasite
Date
2015-10-6
Authors
Bustos, Patricia L.
Perrone, Alina E
Milduberger, Natalia A.
Bua, Jaqueline
Journal Title
Journal ISSN
Volume Title
Publisher
Hilaris
Abstract
Protein samples can be challenging to analyze due to the presence of high-abundance proteins masking low abundance proteins of interest, such as biomarkers and novel physiological mediators. Cyclophilins are chaperones
involved in the cis/trans isomerization of peptidyl-prolyl bonds in peptides or proteins and have been found in every
organism sequenced to date. Although considerable progress has been made in the characterization of some
cyclophilins expressed in diverse parasites invading humans, the main aspects of low-abundance members of this
family remain unknown. In the present work, we present that the combined strategy of using more specific antibodies
and increasing the presence of subcellular proteins in the sample, allowed us to confirm the expression of a 21.1 kDa
cyclophilin for the first time in Trypanosoma cruzi.
Description
Keywords
low-abundance proteins,
subcellular fractionation,
anti-synthetic peptides antibodies,
cyclophilins,
trypanosoma cruzi
Citation
Bustos, P.L.; Perrone, A.E.; Milduberger, N.A.; Bua, J. (2015). Improved immuno-detection of a low-abundance cyclophilin allows the confirmation of its expression in a protozoan parasite. In: Immunochemistry & Immunopathology 1(1):1000103