Browsing Sobre-expresión de ciclofilinas en el parásito unicelular Trypanosoma cruzi: Desarrollo de herramientas para el análisis de la función de proteínas. by Subject "Chagas disease"
(Cell Death Differentiation Association (ADMC), 2017-2-6)
Bustos, Patricia L.; Volta, Viviana J.; Perrone, Alina E; Milduberger, Natalia A.; Bua, Jaqueline
Mitochondria have an important role in energy production, homeostasis and cell death. The opening of the mitochondrial
permeability transition pore (mPTP) is considered one of the key events in apoptosis and necrosis, modulated by cyclophilin D
(CyPD), a crucial component of this protein complex. In Trypanosoma cruzi, the protozoan parasite that causes Chagas disease, we
have previously described that mitochondrial permeability transition occurs after oxidative stress induction in a cyclosporin
A-dependent manner, a well-known cyclophilin inhibitor. In the present work, a mitochondrial parasite cyclophilin, named TcCyP22,
which is homolog to the mammalian CyPD was identified. TcCyP22-overexpressing parasites showed an enhanced loss of
mitochondrial membrane potential and loss of cell viability when exposed to a hydrogen peroxide stimulus compared with control
parasites. Our results describe for the first time in a protozoan parasite that a mitochondrial cyclophilin is a component of the
permeability transition pore and is involved in regulated cell death induced by oxidative stress